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Subject: search.filters.subject.E. COLI

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    Isolation And Molecular Identification Of Escherichia Coli And Salmonella Spp., From Pork, Beef And Chicken Meat Collected From Different Markets In Guaranda, Ecuador
    (Universidad Estatal de Bolívar, 2021-02-13) Bayas Morejón, Favian; Ramón Curay, Riveliño; Olalla García, María; Herrera Chávez, Byron; Carrasco Ruano, Cesar
    The aim of the study was to evaluate the occurrence of E. coli and Salmonella spp. in Sixty-one meat samples (19 chicken, 22 pork and 20 beef), collected from the central markets in Guaranda, Ecuador. All samples were analysed by culture using specific methods for the two genus and the isolates obtained were confirmed by Polymerase Chain Reaction. By culture, 29 samples (47.59%) were positive for Escherichia spp. with 42 isolates, and 25 samples with 35 isolates for Salmonella spp. By PCR, 22 samples (36.07%) were positive for E. coli, and 23 samples (37.70%) for Salmonella spp. The highest prevalence rate of Escherichia coli was observed in pork and beef (16.39% each); A high prevalence of Salmonella spp. was detected in pork (18.03%) followed by beef (13.11%). This study highlights the importance of these pathogens and the need for further studies on its prevalence and distribution in different types of food
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    Detection Of E. Coli By Culture From Human Water Consumption Of Guaranda – Province Of Bolivar (Ecuador)
    (Universidad Estatal de Bolívar, 2022-12-20) Calero García, Mariana; Velarde Escobar, Kristina; Beltrán, Katherin; Herrera Chávez, Renato
    Background: Escherichia coli is a Gram negative opportunistic microorganism, which is directly related to gastroenteritis in depressed immune persons, this pathogen has been found in several biological niches including water; the objective of this work was to detect E. coli by culture techniques. Methods: Fifty samples of water for human consumption in the city of Guaranda, Bolivar (Ecuador) were collected. Cultures were performed using selective media and after incubation under controlled conditions, they were confirmed through biochemical and microscopic tests by Gram stain. Results: After the analysis, 18samples were found to be contaminated with E. coli, which is equivalent to 36% prevalence in the analyzed simples. Conclusion:This contamination level is probably due to the lack of adequate treatments in the water, also the transfer of the fluid and the possible contact with cattle feces in the water springs.