Producción Científica - UEB
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Item Isolation And Molecular Identification Of Escherichia Coli And Salmonella Spp., From Pork, Beef And Chicken Meat Collected From Different Markets In Guaranda, Ecuador(Universidad Estatal de Bolívar, 2021-02-13) Bayas Morejón, Favian; Ramón Curay, Riveliño; Olalla García, María; Herrera Chávez, Byron; Carrasco Ruano, CesarThe aim of the study was to evaluate the occurrence of E. coli and Salmonella spp. in Sixty-one meat samples (19 chicken, 22 pork and 20 beef), collected from the central markets in Guaranda, Ecuador. All samples were analysed by culture using specific methods for the two genus and the isolates obtained were confirmed by Polymerase Chain Reaction. By culture, 29 samples (47.59%) were positive for Escherichia spp. with 42 isolates, and 25 samples with 35 isolates for Salmonella spp. By PCR, 22 samples (36.07%) were positive for E. coli, and 23 samples (37.70%) for Salmonella spp. The highest prevalence rate of Escherichia coli was observed in pork and beef (16.39% each); A high prevalence of Salmonella spp. was detected in pork (18.03%) followed by beef (13.11%). This study highlights the importance of these pathogens and the need for further studies on its prevalence and distribution in different types of foodItem Characterization of natural extracts obtained by supercritical fluids (SCF) from Mashua tubers(Tropaeolum Tuberosum) grown in Ecuador(Universidad Estatal de Bolívar, 2022-06-27) Arellano Gutiérrez, Marco; Herrara Chávez, Byron; Zurita Gallegos, Ronald; Fierro Borja, SoniaThe mashua (Tropaeolum tuberosum) is a plant native to the central Andes whose cultivation is concentrated in Colombia, Bolivia, Ecuador and Peru between 3500 and 4100 meters of altitude, this tuber is rich in nutrients due to its bioactive properties, generally in Ecuador it iscultivated in association with the oca, and the melloco.Therefore, the purpose of this work was to characterize by chromatography two varieties of white and yellow Mashua. Therefore,the supercritical fluid extraction method (FSC) was applied in the two varieties, the volatile compounds of the obtained extractswere characterized by Gas Chromatography coupled to a Mass Spectrometer (GC-MS).After characterization, in general, 16 and 11 major volatile compounds considered to belong to the families or functional groups of ketones, fatty acids, furans, furfurals, alcohols, phyto esters, alkaloids and sterols were identified, some of these compoundsconsidered useful for the prevention of diseases such as cancer, they also have antioxidant properties, hypoglycein agent and in the inhibition of the thyroid gland.