Browsing by Author "Segura Ochoa, Jagger"

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    Specific Detection Of Brucella Spp.In Cattle Slaughtered In The Province Of Bolivar (Ecuador)
    (Universidad Estatal de Bolívar, 2021-12-15) Saltos Ganchala, Ma. José; Ramón Curay, Riveliño; Segura Ochoa, Jagger; Bayas Morejón, Favian
    In this research, we set out to study the prevalence and molecular characterization of Brucella isolates obtained from the blood of cattle slaughtered in 2 herds of the province Bolivar, Ecuador.Therefore, 100 samples of bovine blood were selected, 50 from the Guaranda slaughterhouse and 50 from the Echeandía slaughterhouse.All blood samples were analyzed using the Rose Bengal (RB) serological test, as well as the culture on selective brucella Agar + blood "5% sheep blood supplement" (BAS), under microaerobic conditionsat 37 °C for 24-48 h.Similarly, the isolates obtained by culture were characterized by PCR using a pair of primers that amplify a 905-bp fragment. After the RB serological test, 54% of the analyzed samples were positive, while 63% were positive by culture.Regarding the molecular characterization, 42% of the isolates presented the characteristic band, resulting in being positive for the Brucella genus, being the San Pablo sector the one with the highest prevalence 75%, followed by the Salinas sectorwith 50%, San Lorenzo 47,05% and Echeandía with 31,9%.In relation to the prevalence of the pathogen by sex, this was higher in female cattle.In conclusion, the PCR technique can be considered as a useful molecular tool in the diagnosis of Brucella spp.in cattle, especially due to its greater sensitivity.
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    Susceptibility analysis of Arcobacter isolated from fresh cheeses from municipal markets to fluoroquinolones in Guaranda City, Ecuador.
    (Universidad Estatal de Bolívar, 2022-04-01) Bayas Morejón , Favian; Ramón Curay, Riveliño; Segura Ochoa, Jagger; Martínez, Jenny
    Some species of the Arcobacter genus are considered emerging foodborne enteropathogens. However, the presence of this bacterium in cheese is little known. On the other hand, quinolones are considered first-line drugs for the treatment of campylobacter infection in human patients, but currently little data is available on the levels of resistance to these antibiotics among Arcobacter species. Thus, the objective of this study was to analyze the susceptibility of Arcobacter spp. isolated from 100 fresh cheeses, obtained from the municipal markets to Fluoroquinolones in Guaranda City, Ecuador. By culture, 47 out of the 100 cheese samples were positive with a total of 66 isolates. By PCR, the number of positive samples was reduced to 21 with 26 isolates. With respect to antimicrobial activity, 2 isolates showed resistance to Levofloxacin (7.69%) and 6 to Ciprofloxacin (23.08%). Nineteen strains exhibited intermediate resistance to Levofloxacin and 10 to Ciprofloxacin. This study is the first report on the presence of pathogenic species of Arcobacter spp. in fresh cheeses in Ecuador, which could act as a vehicle for transmission to humans and pose a potential risk to public health.