Browsing by Author "Ramón Curay, Riveliño"

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Asociación de un inmunógeno con un acaricida químico en el control de garrapatas en bovinos en la provincia Pichincha
(Guaranda. Universidad Estatal de Bolívar. Facultad de Ciencias Agropecuarias, Recursos Naturales y del Ambiente. Carrera de Medicina Veterinaria y Zootecnia, 2022) Rodríguez Armijos, Jaime Alexander; Rodríguez Armijos, July Silvana; Ramón Curay, Riveliño
En la investigación desarrollada “Asociación de un inmunógeno con un acaricida químico en el control de garrapatas en bovinos en la provincia pichincha”, se plantearon los siguientes objetivos; establecer la prevalencia de garrapatas adultas presentes en bovinos; comprobar la disminución de garrapatas tras la asociación de un inmunógeno con un acaricida químico; identificar taxonómicamente las principales especies de garrapatas presentes en las fincas y; determinar la infestación de acuerdo a la coloración de pelaje y zona anatómica de los bovinos en estudio. En el marco metodológico se incluyó el material experimental que fueron 80 bovinos, inmunógeno Gavac y acaricidas químicos. También se realizó métodos de laboratorio para la clasificación taxonómica y de campo para el conteo de las garrapatas. Los principales resultados obtenidos fueron qué; la prevalencia de ectoparásitos en promedio general se registró en un 97.5% de la población total. En el conteo inicial de garrapatas, en promedio general existieron 67 ácaros/bovino; mientras que al final del ensayo se cuantificó 23 garrapatas/bovino. La mejor respuesta en relación de la reducción del número de ectoparásitos a través del tiempo, se determinó en la combinación del inmunógeno (GAVAC ™) en una dosis de 2 ml + baño por aspersión cada 15 días con Amitraz en una dosis de 20ml/20 lts de agua. Se identificó una sola especie de ectoparásitos (Rhipicephalus microplus). La zona anatómica del bovino con mayor prevalencia de garrapatas, fueron los miembros posteriores. Existió una predisposición de mayor infestación en el color oscuro.
Isolation And Molecular Identification Of Escherichia Coli And Salmonella Spp., From Pork, Beef And Chicken Meat Collected From Different Markets In Guaranda, Ecuador
(Universidad Estatal de Bolívar, 2021-02-13) Bayas Morejón, Favian; Ramón Curay, Riveliño; Olalla García, María; Herrera Chávez, Byron; Carrasco Ruano, Cesar
The aim of the study was to evaluate the occurrence of E. coli and Salmonella spp. in Sixty-one meat samples (19 chicken, 22 pork and 20 beef), collected from the central markets in Guaranda, Ecuador. All samples were analysed by culture using specific methods for the two genus and the isolates obtained were confirmed by Polymerase Chain Reaction. By culture, 29 samples (47.59%) were positive for Escherichia spp. with 42 isolates, and 25 samples with 35 isolates for Salmonella spp. By PCR, 22 samples (36.07%) were positive for E. coli, and 23 samples (37.70%) for Salmonella spp. The highest prevalence rate of Escherichia coli was observed in pork and beef (16.39% each); A high prevalence of Salmonella spp. was detected in pork (18.03%) followed by beef (13.11%). This study highlights the importance of these pathogens and the need for further studies on its prevalence and distribution in different types of food
Specific Detection Of Brucella Spp.In Cattle Slaughtered In The Province Of Bolivar (Ecuador)
(Universidad Estatal de Bolívar, 2021-12-15) Saltos Ganchala, Ma. José; Ramón Curay, Riveliño; Segura Ochoa, Jagger; Bayas Morejón, Favian
In this research, we set out to study the prevalence and molecular characterization of Brucella isolates obtained from the blood of cattle slaughtered in 2 herds of the province Bolivar, Ecuador.Therefore, 100 samples of bovine blood were selected, 50 from the Guaranda slaughterhouse and 50 from the Echeandía slaughterhouse.All blood samples were analyzed using the Rose Bengal (RB) serological test, as well as the culture on selective brucella Agar + blood "5% sheep blood supplement" (BAS), under microaerobic conditionsat 37 °C for 24-48 h.Similarly, the isolates obtained by culture were characterized by PCR using a pair of primers that amplify a 905-bp fragment. After the RB serological test, 54% of the analyzed samples were positive, while 63% were positive by culture.Regarding the molecular characterization, 42% of the isolates presented the characteristic band, resulting in being positive for the Brucella genus, being the San Pablo sector the one with the highest prevalence 75%, followed by the Salinas sectorwith 50%, San Lorenzo 47,05% and Echeandía with 31,9%.In relation to the prevalence of the pathogen by sex, this was higher in female cattle.In conclusion, the PCR technique can be considered as a useful molecular tool in the diagnosis of Brucella spp.in cattle, especially due to its greater sensitivity.
Study Of The Antibacterial Activity Of Extracts And Oil Of Uvilla (Physalis Peruviana L) On Strains Of Escherichia Coli, Salmonella Spp And Arcobacter Spp In Ecuador
(Universidad Estatal de Bolívar, 2021-11-07) Bayas Morejón, Favian; Ramón Curay, Riveliño; Beltrán Moso, Katherin; Moso Ortiz, Mary
Resistance of pathogenic bacteria to multiple antimicrobials has become a global threat to health and food safety. This research determined the antimicrobial activity of extracts and oils of Physalis peruviana, where it was established as study factors: extracts of parts of the plant (leaves and stem) and oils of the uvilla berry (using soxhlet); for antimicrobial activity, Escherichia, Salmonella and Arcobacter strains belonging to the microorganism bank of the State University of Bolivar were used. After the analysis, the stem extract showed a better inhibitory effect against Escherichia coli strains with a halo diameter of 14,67 mm, a value very close to that produced by streptomycin and penicillin; with respect to the Salmonella strains, the best effect was presented by the oil of the Physalis peruviana berry with 14 mm, in this case, the antibiotics for clinical use presented values greater than 20 mm. Finally, for Arcobacter the results were not encouraging, being only the extract of the leaves the one that presented activity of 3,5 mm, well below that established to be considered an antibiotic agent. Therefore, it is concluded that the analyzed uvilla extracts and oils are effective against γ proteo bacteria, but not against ε proteo bacteria.
Susceptibility analysis of Arcobacter isolated from fresh cheeses from municipal markets to fluoroquinolones in Guaranda City, Ecuador.
(Universidad Estatal de Bolívar, 2022-04-01) Bayas Morejón , Favian; Ramón Curay, Riveliño; Segura Ochoa, Jagger; Martínez, Jenny
Some species of the Arcobacter genus are considered emerging foodborne enteropathogens. However, the presence of this bacterium in cheese is little known. On the other hand, quinolones are considered first-line drugs for the treatment of campylobacter infection in human patients, but currently little data is available on the levels of resistance to these antibiotics among Arcobacter species. Thus, the objective of this study was to analyze the susceptibility of Arcobacter spp. isolated from 100 fresh cheeses, obtained from the municipal markets to Fluoroquinolones in Guaranda City, Ecuador. By culture, 47 out of the 100 cheese samples were positive with a total of 66 isolates. By PCR, the number of positive samples was reduced to 21 with 26 isolates. With respect to antimicrobial activity, 2 isolates showed resistance to Levofloxacin (7.69%) and 6 to Ciprofloxacin (23.08%). Nineteen strains exhibited intermediate resistance to Levofloxacin and 10 to Ciprofloxacin. This study is the first report on the presence of pathogenic species of Arcobacter spp. in fresh cheeses in Ecuador, which could act as a vehicle for transmission to humans and pose a potential risk to public health.